Two procedures compared for determination of plasma cholinesterase activity.

Determination of cholinesterase activity in human blood.

A new high activity plasma cholinesterase variant.

A South African Afrikaans speaking family is reported in which a new high activity plasma cholinesterase variant was found to occur in the mother and son. The variant has the same electrophoretic mobility as the "usual' enzyme, but greater heat stability. Its higher specific activity is associated with a normal number of enzyme molecules. The variant may be inherited as a dominant trait, though...

Plasma Cholinesterase Activity in Alzheimer's Disease.

Cholinesterase inhibitors (ChEIs) are not allowed to be prescribed in combination, which means that we need to select 1 of 3 ChEIs for use in a patient with Alzheimer's disease (AD). However, there is no quantitative analysis on the differences between these agents. In this article, we propose that plasma cholinesterase activity (pChE) could be used as the standard for differentiating between r...

Spectrophotometric micromethod for measuring cholinesterase activity in serum or plasma.

A simple ultraviolet method has been developed for determining serum or plasma cholinesterase activity. The cholinesterase acts on acetylor butyrylthiocholine to release thiocholine, which reacts with 2,2’or 4,4’-dithiodipyridine, showing absorbance at 324 or 343 nm, respectively. The activity can be measured kinetically and alsoafter incubation for 3 to 5 mm at 20#{176} to 37#{176}C. A volume ...

Comparison of a commercially available assay system with two reference methods for the determination of plasma cholinesterase variants.

For assaying plasma cholinesterase (EC 3.1.1.8) activity and phenotyping by means of dibucaine inhibition, we have compared a commercially available kit, in which butyrylthiocholine is used as substrate, with two reference methods, one using benzoylcholine and the other propionylthiocholine. With 50 different samples of three of the most common genetic variants, we could clearly differentiate t...